Journal: Acta biomaterialia
Article Title: Nanoparticles of alkylglyceryl-dextran-graft-poly(lactic acid) for drug delivery to the brain: Preparation and in vitro investigation.
doi: 10.1016/j.actbio.2015.05.009
Figure Lengend Snippet: Fig. 9. Electric Cell-substrate Impedance Sensing (ECIS) plot of the variation of TEER values (as %, relative to control) at 2000 Hz using (A) bEnd3 and (B) hCMEC/D3 brain endothelial cell monolayers cultured on 8W10E test plates, following exposure to and then removal of nanoparticles(timepoints indicated by orange arrows) prepared from PLA-DEX and PLA-DEX-OX4, in dose–response experiments. Nanoformulations were added to the cells at 48 h and removed at 72 h, after which the cells were allowed to recover for a further 48 h. Data presented as means of three wells (n = 3), for each condition of a representative measurement (error bars represent ±SD). Untreated cells (PBS, no nanoparticles added) were employed as control.
Article Snippet: Electric Cell-substrate Impedance Sensing (ECIS) measurements were conducted with an ECIS Zh instrument (Applied Biophysics) operating at a frequency range of 2–32 kHz and using 8 well-arrays type 8W10E.
Techniques: Electric Cell-substrate Impedance Sensing, Control, Cell Culture